A real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA) was developed based on the nucleocapsid gene of PEDV. RT-RPA assay was performed at 40 °C for 20 min. The assay could detect both the classical and variant PEDV strains, and there was no cross-reaction with other pathogens tested.
In this study, we describe the development of a reverse transcription isothermal Recombinase Polymerase Amplification (RT-RPA) assay for the identification of MERS-CoV. A partial nucleocapsid gene RNA molecular standard of MERS-coronavirus was used to determine the assay sensitivity.
Characterization and a RT-RPA assay for rapid detection of Chilli Veinal mottle virus (ChiVMV) in tobacco. The characterization of ChiVMV-LZ infecting tobacco was determined, and the established RT-RPA assay provides a reliable and effective method for rapid detection of ChiVMV in tobacco.
3/10/2020 · The genome sequence of ChiVMV-LZ was characterized by sequence alignment and phylogenetic analysis. Then, a RT-RPA assay was established for rapid and sensitive detection of ChiVMV-LZ in tobacco. Additionally, the established RT-RPA assay was compared to the RT-PCR assay in aspect of sensitivity and application in field-collected tobacco samples.
7/5/2019 · Diagnostic sensitivity of the RT-RPA assay. The RT-RPA assay was evaluated by testing a cohort of rabies positive and negative samples and compared to qRT-PCR . All samples were detected with qRT-PCR and the RT-RPA resulting in a diagnostic sensitivity of 100%. The average detection time of the RT-RPA was 5.3 minutes compared to 47.6 minutes for qRT-PCR.
Reverse transcription recombinase polymerase amplification …
Characterization and a RT- RPA assay for rapid detection of …
Characterization and a RT- RPA assay for rapid detection of …
Recombinase polymerase amplification – Wikipedia, 2/4/2021 · Herein, we developed a reverse transcription recombinase polymerase amplification ( RT-RPA) assay as potential point-of-need diagnostic tool for.
6/15/2015 · The RT-RPA assay was tested in a mobile laboratory combining magnetic-bead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38°C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and.
A probe-based reverse transcription RPA (RT-RPA) assay was developed for real-time detection of PDCoV. The amplification can be finished in 20 min and fluorescence monitoring was performed by a portable device.
real-time RT-RPA (B). Both assays have a sensitivity of 10 RNA molecules. The RT-RPA assay was much faster than the RT-PCR as the run time of the RT-RPA is between 3-7 minutes for 10 7 and 10 1 molecules, respectively. While the RT-PCR needed up to 2 hours. Consequently, RT-PCR results is linear, while RT-RPA not. PLOS Currents Outbreaks 7
